The Biocrates bile acid panel measures the concentration of 20 different bile acids. Total bile acid (TBA) level was calculated by summation of 15 conjugated and unconjugated primary and secondary BA levels. We also used Biocrates AbsoluteIDQ® p150 kit (BIOCRATES Life Sciences AG, . Samples: serum, plasma, urine and other biological samples. Sulfation of bile acids increases their solubility and decreases intestinal absorption, thereby enhancing fecal and urinary excretion. Bile acid quantification was performed by the Sick Kids Analytical Facility for Molecules (AFBM) by liquid chromatography tandem mass spectrometry (LC-MS-MS) using the Biocrates® Life Sciences Bile Acids Kit (Biocrates, Innsbruck, Austria) according to the manufacturer’s instructions. [16]. Read user reviews, compare products & request pricing of BIOCRATES Life Sciences AG - Cell-Based Assays laboratory products & instrumentation. It provides metabolic phenotyping services. Cholic acid AbsoluteIDQ p180 (188) (Kit / Service) Our most frequently used targeted metabolomics discovery panel. Bile acids are essential for regulating cholesterol metabolism and the intestinal digestion and absorption of fat. Bile Acid quantification – Biocrates AbsoluteIDQ - Bile Acids Kit. Quantification of 20 steroids is done using Biocrates AbsoluteIDQTM technology. Biocrates ® Bile Acids Kit - primary bile acids - secondary bile acids - human and rodent specific bile acids. 188 metabolites, which were ca-refully selected based on studies of the human blood metabolome. Objectives: The aim of the present study is to measure concentrations of 20 bile acids using the novel Kit from Biocrates Life Sciences based on LC-MS technique. There was no difference in incremental AUC 0–24 between the tolerant and intolerant cohorts for each individual bile acid, when corrected for multiple testing. BA quantification was performed by liquid chromatography tandem mass spectrometry using the Biocrates Life Sciences Bile Acids Kit (BIOCRATES Life Science AG, Innsbruck, Austria) according to manufacturer's instructions (see Table 3 for list of BAs, abbreviations, and their levels across diagnosis groups). Samples were prepared using the Bile Acids kit (Biocrates Innsbruck, Austria) in strict accordance with their detailed protocol. Objectives The aim of the present study is to measure concentrations of 20 bile acids using the novel Kit from Biocrates Life Sciences based on LC-MS technique. Bile acids were analyzed using the commercially available Biocrates Bile Acids Kit (Biocrates Life Science AG, Innsbruck, Austria) as described by Marksteiner et al. Bile acids are essential for regulating cholesterol metabolism and the intestinal digestion and absorption of fat. Laboratories were first required to pass the acceptance criteria for the SST. Addition of 10 µL of the supplied internal standard solution to each well of the 96-well extraction plate was followed by drying under a gentle stream of nitrogen. Fecal BAs were assessed by electrospray (ESI) mass spectrometry coupled to liquid chromatography (LC) with a tandem mass spectrometry (MS/MS) instrument (AB SCIEX, ThermoScientific, Waters) using a kit customized by Biocrates® (Biocrates® Bile Acids Kit), which enabled the absolute quantification of up to 20 different BA. Hartmut Glossmann is Co-Founder at BIOCRATES Life Sciences AG. Cookie Type Duration Description; fpestid: 0: 1 year: Fpestid is a ShareThis cookie ID set in the domain of the website operator. The MxP Quant 500 kit combines LC-MS/MS of 13 compound classes, basically small molecules, bile acids, and free fatty acids, followed by FIA-MS/MS of 12 lipid classes (including acylcarnitines) and hexoses, into a single workflow (Figure 1). Bile acids (BAs), products of cholesterol metabolism and clearance, are produced in the … Samples were processed according to the manufacturer’s instructions. The synthesis of primary bile acids, and the conjugation of bile acids with tauring and glycine, takes place in the liver. The BIOCRATES® Bile Acids Kit* is based on (ultra) high performance liquid chromatography coupled with electrospray tandem mass spectrometry (UHPLC-MS/MS) and is characterized by quick and highly effective sample extraction on a 96-well patented filter plate. The bile acid kit provides simultaneous quantification of 22 bile acids (properties outlined in Table 6). Bile acid is metabolized in the liver and is present in increased concentrations with abnormal liver function. Liquid handling is done on ML Star Hamilton robot. The Biocrates® Bile Acids Kit is the fi rst ready to use solution to quantify 20 bile acids in a standardized and high throughput fashion. Read user reviews, compare products & request pricing of BIOCRATES Life Sciences AG - ADME-Toxicology laboratory products & instrumentation. The complete analytical process (Data processing, sample registration, work list generation) was accomplished using the MetIDQ™ software package, which is an integral part of the Biocrates ® Bile Acid Kit. The nomeclature has been proposed by Hofmann et al. Metabolomics (Bile Acids) Created By Mette Peters Mette The objective was to measure the levels of selected bile acids in 159 human plasma and serum samples using the Biocrates Bile Acids kit. spectrometer (Waters Inc., Milford, Massachusetts, USA). Each laboratory received a Biocrates ® Bile Acids Kit including system suitability test (SST) protocol. The lysine degradation product 2-aminoadipic acid is heavily decreased in concentration which already hints at a protective effect according to the authors. The Biocrates Bile Acids assay quantifies 20 bile acids, 16 of which are normally detected in human plasma. BioAssay Systems' Bile Acid Assay Kit (EFBA-100) provides a fluorimetric means to measure total bile acids. Quantification of bile acids in both brain tissue extract and plasma were performed using the Biocrates Bile Acids kit (BIOCRATES, Life Science AG, Innsbruck, Austria). Fluorescence intensity (530/585 nm) is linear to the bile acid concentration in the sample. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, bile acid standards and unknown samples. Biocrates© Bile Acids Kit (96 wells) - Waters for up to 84 samples; for HPLC and UHPLC pump System Appelez-nous : +33 (0)2.32.64.45.45 Liste des fabricants Site instrumentation The major bile acid is cholic acid, which can be conjugated or unconjugated forming different isoforms: deoxy-glyco-cheno-lith-hyo-tauro or urso. BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria. Bile acids are an important test for monitoring normal liver function. App Note. BIOCRATES Life Sciences, an Austrian developer of mass spectrometry-based kits, has launched the first ever LC-MS assay for determining endogenous bile acids. Detection range: 1 - 150 µM bile acids. The novel Bile Acid kit developed by Biocrates allows to measure 20 bile acid metabolites with very high sensitivity (up to 3 nM). A standardized quantitative Kit developed that allows the simultaneous analysis of 20 bile acids in 20 μL sample volume. Bile Acid Kit (20) (Kit / Service) Bile acids are important indicators of the hepato-biliary function as well as the gut microbiome. Acute heart failure is characterized by a heavily reorganized metabolic phenotype especially in amino acids and lipids in … locale: session: 1 month: Used by the site to show content to users based on their selected location. Austrian BIOCRATES Life Sciences AG, internationally renowned developer and marketer of innovative targeted metabolomics solutions, further expands its range of metabolomics-based An injection volume of 10 μL was used. The kit is designed to analyze 16 individual human and 19 mouse bile acids. Biocrates Bile Acids. Their signaling functions play an important role in chronic disorders, sepsis, or Introduction: Increasing evidence suggests a role for the gut microbiome in central nervous system disorders and a specific role for the gut-brain axis in neurodegeneration. Kairos Amino Acid 6. The microbiota induces modifi cations (dehydroxylation and/or decon- BIOCRATES Life Sciences AG, developer of cutting-edge mass-spectrometry-based kit products, has launched the first-ever bile acids kit based on targeted metabolite quantitation. This assay can be used to measure bile acid concentrations in mice in a wide variety of applications including mouse serum, liver, bile, feces, and intestine. 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